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microchannel acoustophoresis does not impact survival or function of microglia, leukocytes or tumor cells
background the use of acoustic forces to manipulate particles or cells at the microfluidic scale (i.e. acoustophoresis), enables non-contact, label-free separation based on intrinsic cell properties such as size, density and compressibility. acoustophoresis holds great promise as a cell separation technique in several research and clinical areas. however, it has been suggested that the force acting upon cells undergoing acoustophoresis may impact cell viability, proliferation or cell function via subtle phenotypic changes. if this were the case, it would suggest that the acoustophoresis method would be a less useful tool for many cell analysis applications as well as for cell therapy. methods we investigate, for the first time, several key aspects of cellular changes following acoustophoretic processing. we used two settings of ultrasonic actuation, one that is used for cell sorting (10 vpp operating voltage) and one that is close to the maximum of what the system can generate (20 vpp). we used microglial cells and assessed cell viability and proliferation, as well as the inflammatory response that is indicative of more subtle changes in cellular phenotype. furthermore, we adapted a similar methodology to monitor the response of human prostate cancer cells to acoustophoretic processing. lastly, we analyzed the respiratory properties of human leukocytes and thrombocytes to explore if acoustophoretic processing has adverse effects. results bv2 microglia were unaltered after acoustophoretic processing as measured by apoptosis and cell turnover assays as well as inflammatory cytokine response up to 48 h following acoustophoresis. similarly, we found that acoustophoretic processing neither affected the cell viability of prostate cancer cells nor altered their prostate-specific antigen secretion following androgen receptor activation. finally, human thrombocytes and leukocytes displayed unaltered mitochondrial respiratory function and integrity after acoustophoretic processing. conclusion we conclude that microchannel acoustophoresis can be used for effective continuous flow-based cell separation without affecting cell viability, proliferation, mitochondrial respiration or inflammatory status.
a microfluidic platform for precision small-volume sample processing and its use to size separate biological particles with an acoustic microdevice
lawrence livermore national laboratory. this protocol describes a system architecture for performing automated small volume (0.15–1.5 ml) particle separations using a microfluidic device, and discusses methods to optimize acoustofluidic device performance and operation.
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electrochemically etched tapered-tip stainless-steel electrospray-ionization emitters for capillary electrophoresis–mass spectrometry
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do you prefer a rotary tool or engraver for etching designs in wood/metal?
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microchip capillary electrophoresis
leading chemists and engineers concisely explain the principles behind microchip capillary electrophoresis and demonstrate its use in a variety of biochemical applications, ranging from the analysis of dna, proteins, and peptides to single cell analysis and measuring the impact of surface modification on flow in microfluidic channels. since surface chemistry must be carefully considered for optimal operation at this scale, the authors also discuss methods of both adsorbed and covalent surface modification for its control. fabrication methods for producing microchips with glass, poly(dimethylsiloxane), and other polymers are also provided so that even novices can produce simple devices for standard separations. microchip capillary electrophoresis: methods and protocols provides a practical starting point for either initiating research in the field of microchip capillary electrophoresis or understanding the full range of what can be done with existing systems.
apex custom concrete & masonry : apex custom concrete & masonry: amazon.in: किताबें
apex custom concrete & masonry : apex custom concrete & masonry: amazon.in: किताबें
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